RGD Reference Report - Mass spectrometric characterization of mitochondrial electron transport complexes: subunits of the rat heart ubiquinol-cytochrome c reductase. - Rat Genome Database

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Mass spectrometric characterization of mitochondrial electron transport complexes: subunits of the rat heart ubiquinol-cytochrome c reductase.

Authors: Sun, G  Kinter, MT  Anderson, VE 
Citation: Sun G, etal., J Mass Spectrom 2003 May;38(5):531-9.
RGD ID: 1299349
Pubmed: PMID:12794875   (View Abstract at PubMed)
DOI: DOI:10.1002/jms.467   (Journal Full-text)

Complex III of the mitochondrial electron transport chain, ubiquinol-cytochrome c reductase, was isolated by blue native polyacrylamide gel electrophoresis. Ten of the 11 polypeptides present in this complex were detected directly by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) following electroelution of the active complex. Tryptic and chymotryptic digestion of the complex permit the identification of specific peptides from all of the protein subunits with 70% coverage of the 250 kDa complex. The mass of all 11 proteins was confirmed by second dimension Tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and elution of the separated polypeptides. Additionally, the identity of the core I, core II, cytochrome c and the Rieske iron-sulfur protein were confirmed by liquid chromatography/tandem mass spectrometry (LC/MS/MS) characterization of the peptides generated by in-gel trypsin digestion of the SDS-PAGE separated proteins. The methodology demonstrated for analyzing this membrane-bound electron transport complex should be applicable to other membrane complexes, particularly the other mitochondrial electron transport complexes. The MS analysis of the peptides obtained by in-gel digestion of the intact complex permits the simultaneous characterization of the native proteins and modifications that contribute to mitochondrial deficits that have been implicated as contributing to pathological conditions.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
UqcrbRatrespiratory chain complex III  IDA Uqcrb is subunit VIIRGD 
Uqcrc1Ratrespiratory chain complex III  IDA Uqcrc1 is subunit IRGD 
Uqcrc2Ratrespiratory chain complex III  IDA Uqcrc2 is subunit IIRGD 
Uqcrfs1Ratrespiratory chain complex III  IDA Uqcrfs1 is subunit VRGD 
UqcrhRatrespiratory chain complex III  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
UqcrbRatprotein-containing complex binding  IDA heterooligomerization and Uqcrb is subunit VIIRGD 
Uqcrc1Ratprotein-containing complex binding  IDA  RGD 
UqcrhRatprotein-containing complex binding  IDA heterooligomerization and Uqcrh is subunit VIIIRGD 

Objects Annotated

Genes (Rattus norvegicus)
Uqcrb  (ubiquinol-cytochrome c reductase binding protein)
Uqcrc1  (ubiquinol-cytochrome c reductase core protein 1)
Uqcrc2  (ubiquinol cytochrome c reductase core protein 2)
Uqcrfs1  (ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1)
Uqcrh  (ubiquinol-cytochrome c reductase hinge protein)


Additional Information