RGD Reference Report - Autophosphorylation of cGMP-dependent protein kinase type II. - Rat Genome Database

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Autophosphorylation of cGMP-dependent protein kinase type II.

Authors: Vaandrager, AB  Hogema, BM  Edixhoven, M  Van den Burg, CM  Bot, AG  Klatt, P  Ruth, P  Hofmann, F  Van Damme, J  Vandekerckhove, J  De Jonge, HR 
Citation: Vaandrager AB, etal., J Biol Chem 2003 Aug 1;278(31):28651-8. Epub 2003 May 22.
RGD ID: 1299018
Pubmed: PMID:12764134   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M303699200   (Journal Full-text)

Cyclic nucleotides are shown to stimulate the autophosphorylation of type II cGMP-dependent protein kinase (cGK) on multiple sites. Mass spectrometric based analyses, using a quadrupole time-of-flight-mass spectrometry instrument revealed that cGMP stimulated the in vitro phosphorylation of residues Ser110 and Ser114, and, at a slow rate, of Ser126 and Thr109 or Ser117, all located in the autoinhibitory region. In addition Ser445 was found to be phosphorylated in a cGMP-dependent manner, whereas Ser110 and Ser97 were already prephosphorylated to a large extent in Sf9 cells. cGMP-dependent phosphorylation of cGK II was also demonstrated in intact COS-1 cells and intestinal epithelium. Substitution of most of the potentially autophosphorylated residues for alanines largely abolished the cGMP stimulation of the autophosphorylation. Prolonged autophosphorylation of purified recombinant cGK II in vitro resulted in a 40-50% increase in basal kinase activity, but its maximal cGMP-stimulated activity and the EC50 for cGMP remained unaltered. Mutation of the major phosphorylatable serines 110, 114, and 445 into "phosphorylation-mimicking" glutamates had no effect on the kinetic parameters of cGK II. However, replacing the slowly autophosphorylated residue Ser126 by Glu rendered cGK II constitutively active. These results show that the fast phase of cyclic nucleotide-stimulated autophosphorylation of cGK II has a relatively small feed forward effect on its activity, whereas the secondary phase, presumably involving Ser126 phosphorylation, may generate a constitutively active form of the enzyme.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
peptidyl-serine autophosphorylation  IDA 1299018 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Prkg2  (protein kinase cGMP-dependent 2)

Additional Information