RGD Reference Report - Contribution of extrathymic gamma delta T cells to the expression of heat-shock protein and to protective immunity in mice infected with Toxoplasma gondii. - Rat Genome Database

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Contribution of extrathymic gamma delta T cells to the expression of heat-shock protein and to protective immunity in mice infected with Toxoplasma gondii.

Authors: Hisaeda, H  Sakai, T  Nagasawa, H  Ishikawa, H  Yasutomo, K  Maekawa, Y  Himeno, K 
Citation: Hisaeda H, etal., Immunology. 1996 Aug;88(4):551-7.
RGD ID: 12910478
Pubmed: PMID:8881756   (View Abstract at PubMed)
PMCID: PMC1456633   (View Article at PubMed Central)

We demonstrated that gamma delta T cells contribute to protective immunity against Toxoplasma gondii by inducing the expression of a 65,000 MW heat-shock protein (hsp 65) in host macrophages. Here we examined the role of extrathymic and intrathymic gamma delta T cells in protective immunity and hsp 65 expression in mice infected with T. gondii. Intrathymic gamma delta T cells were obtained from severe combined immunodeficiency (SCID) mice grafted with syngeneic fetal thymus (TG-SCID), in which only T cells derived from the donor thymus developed, whereas extrathymic gamma delta T cells were obtained from nude mice that lack thymus. Extrathymic gamma delta T cells from T. gondii-infected nude mice differed from intrathymic gamma delta T cells of infected TG-SCID mice, in terms of Thy1.2 expression and V-region gene usage of T-cell receptor (TCR) gamma delta. Extrathymic gamma delta T cells expressed extremely high levels of Thy1.2, and had V gamma 7 repertoire but lacked V gamma 5,6 and V delta 1,5. On the other hand, intrathymic gamma delta T cells express intermediate and low levels of Thy1,2. These cells possessed V gamma 5,6 and V delta 1,5 but failed to rearrange the V gamma 7 gene. Peritoneal macrophages from infected nude mice contained hsp 65, whereas this protein was scarcely expressed in those of infected TG-SCID mice. Transfer of extrathymic, but not of intrathymic gamma delta T cells to SCID mice enabled their macrophages to express hsp 65. Athymic nude mice were significantly resistant to the infection compared with SCID mice which lack gamma delta T as well as alpha beta T cells. The resistance was dependent upon extrathymic gamma delta T cells, since nude mice depleted of gamma delta T cells using a corresponding monoclonal antibody became extremely susceptible. These results indicated that extrathymic rather than intrathymic gamma delta T cells play some crucial roles in protection against T. gondii and in hsp 65 expression.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
HSPD1HumanAnimal Toxoplasmosis  ISOHspd1 (Mus musculus) RGD 
Hspd1RatAnimal Toxoplasmosis  ISOHspd1 (Mus musculus) RGD 
Hspd1MouseAnimal Toxoplasmosis  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hspd1  (heat shock protein family D (Hsp60) member 1)

Genes (Mus musculus)
Hspd1  (heat shock protein 1 (chaperonin))

Genes (Homo sapiens)
HSPD1  (heat shock protein family D (Hsp60) member 1)


Additional Information