RGD Reference Report - Creation and preliminary characterization of a leptin knockout rat. - Rat Genome Database

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Creation and preliminary characterization of a leptin knockout rat.

Authors: Vaira, Sergio  Yang, Chang  McCoy, Aaron  Keys, Kelly  Xue, Shurong  Weinstein, Edward J  Novack, Deborah V  Cui, Xiaoxia 
Citation: Vaira S, etal., Endocrinology. 2012 Nov;153(11):5622-8. doi: 10.1210/en.2012-1462. Epub 2012 Sep 4.
RGD ID: 12904911
Pubmed: (View Article at PubMed) PMID:22948215
DOI: Full-text: DOI:10.1210/en.2012-1462

Leptin, a cytokine-like hormone secreted mainly by adipocytes, regulates various pathways centered on food intake and energy expenditure, including insulin sensitivity, fertility, immune system, and bone metabolism. Here, using zinc finger nuclease technology, we created the first leptin knockout rat. Homozygous leptin null rats are obese with significantly higher serum cholesterol, triglyceride, and insulin levels than wild-type controls. Neither gender produced offspring despite of repeated attempts. The leptin knockout rats also have depressed immune system. In addition, examination by microcomputed tomography of the femurs of the leptin null rats shows a significant increase in both trabecular bone mineral density and bone volume of the femur compared with wild-type littermates. Our model should be useful for many different fields of studies, such as obesity, diabetes, and bone metabolism-related illnesses.

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Objects Annotated

Genes (Rattus norvegicus)
Lep  (leptin)
Lepem1Sage  (leptin; zinc finger nuclease induced mutant1, Sage)

Genes (Mus musculus)
Lep  (leptin)

Genes (Homo sapiens)
LEP  (leptin)

Objects referenced in this article
Gene SH2B3 SH2B adaptor protein 3 Homo sapiens
Gene Sh2b3 SH2B adaptor protein 3 Mus musculus
Gene Sh2b3 SH2B adaptor protein 3 Rattus norvegicus
Gene Sh2b3em2Mcwi SH2B adaptor protein 3; zinc finger nuclease induced mutant 2, Medical College of Wisconsin Rattus norvegicus
Strain SS-Sh2b3em2Mcwi-/- SS-Sh2b3em2Mcwi-/Sh2b3em2Mcwi- Rattus norvegicus

Additional Information