RGD Reference Report - Mitochondrial expression of a short dystrophin-like product with molecular weight of 71 kDa. - Rat Genome Database

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Mitochondrial expression of a short dystrophin-like product with molecular weight of 71 kDa.

Authors: Chávez, O  Harricane, M C  Alemán, V  Dorbani, L  Larroque, C  Mornet, D  Rendon, A  Martínez-Rojas, D 
Citation: Chávez O, etal., Biochem Biophys Res Commun. 2000 Aug 2;274(2):275-80.
RGD ID: 12880018
Pubmed: PMID:10913331   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.2000.3118   (Journal Full-text)

In the brain, Dp71 is the most abundant protein product of the DMD gene and by alternative splicing of exon 78 two isoforms can be expressed, Dp71d and Dp71f. To explore the subcellular distribution of these Dp71 isoforms, specific monoclonal antibodies were used. Dp71d (with exon 78) was found in microsomes, while Dp71f (without exon 78) was detected in mitochondria. To determine the alterations which the absence of dystrophin proteins induces, we compared the expression of Dp71d in microsomes and Dp71f in mitochondria from mdx and mdx(3CV) mice. Dp71d in microsomes of mdx was similar to that of wild-type mice and, as expected, in mdx(3CV) this protein was undetectable. However, in mitochondria from mdx(3CV), Dp71f was overexpressed in comparison to mitochondria from mdx mice. Because in mdx(3CV) mice all the dystrophin proteins are mutated or diminished, we concluded that the protein detected in mitochondria is not a Dp71f but a novel product named Dp71f-like protein.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
DmdRatmatrix side of mitochondrial inner membrane  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dmd  (dystrophin)


Additional Information