RGD Reference Report - Congenic mapping and sequence analysis of the Renin locus. - Rat Genome Database

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Congenic mapping and sequence analysis of the Renin locus.

Authors: Flister, Michael J  Hoffman, Matthew J  Reddy, Prajwal  Jacob, Howard J  Moreno, Carol 
Citation: Flister MJ, etal., Hypertension. 2013 Apr;61(4):850-6. doi: 10.1161/HYPERTENSIONAHA.111.01008. Epub 2013 Mar 4.
RGD ID: 12802369
Pubmed: PMID:23460292   (View Abstract at PubMed)
PMCID: PMC3770301   (View Article at PubMed Central)
DOI: DOI:10.1161/HYPERTENSIONAHA.111.01008   (Journal Full-text)

Renin was the first blood pressure (BP) quantitative trait locus mapped by linkage analysis in the rat. Subsequent BP linkage and congenic studies capturing different portions of the renin region have returned conflicting results, suggesting that multiple interdependent BP loci may be residing in the chromosome 13 BP quantitative trait locus that includes Renin. We used SS-13(BN) congenic strains to map 2 BP loci in the Renin region (chr13: 45.2-49.0 Mb). We identified a 1.1-Mb protective Brown Norway region around Renin (chr13: 46.1-47.2 Mb) that significantly decreased BP by 32 mm Hg. The Renin protective BP locus was offset by an adjacent hypertensive locus (chr13: 47.2-49.0 Mb) that significantly increased BP by 29 mm Hg. Sequence analysis of the protective and hypertensive BP loci revealed 1433 and 2063 variants between Dahl salt-sensitive/Mcwi and Brown Norway rats, respectively. To further reduce the list of candidate variants, we regenotyped an overlapping SS-13(SR) congenic strain (S/renrr) with a previously reported BP phenotype. Sequence comparison among Dahl salt-sensitive, Dahl R, and Brown Norway reduced the number of candidate variants in the 2 BP loci by 42% for further study. Combined with previous studies, these data suggest that at least 4 BP loci reside within the 30-cM chromosome 13 BP quantitative trait locus that includes Renin.

Disease Annotations    
hypertension  (IAGP,ISO)

Phenotype Annotations    

Mammalian Phenotype

Objects Annotated

Genes (Rattus norvegicus)
Chi3l1  (chitinase 3 like 1)
Chit1  (chitinase 1)
Etnk2  (ethanolamine kinase 2)
Gpr37l1  (G protein-coupled receptor 37-like 1)
Ipo9  (importin 9)
Lgr6  (leucine-rich repeat-containing G protein-coupled receptor 6)
Mybph  (myosin binding protein H)
Plekha6  (pleckstrin homology domain containing A6)
Ptprv  (protein tyrosine phosphatase, receptor type, V)
Rnpep  (arginyl aminopeptidase)
Sox13  (SRY-box transcription factor 13)
Syt2  (synaptotagmin 2)

Genes (Mus musculus)
Mybph  (myosin binding protein H)

Genes (Homo sapiens)
MYBPH  (myosin binding protein H)

Bp395  (Blood pressure QTL 395)
Bp396  (Blood pressure QTL 396)

Objects referenced in this article
Marker RN34_13048990782 RN34_13048990782 Rattus norvegicus
Marker 2340 2340 Rattus norvegicus
Marker 4582 4582 Rattus norvegicus
QTL Bp400 Blood pressure QTL 400 Rattus norvegicus

Additional Information