RGD Reference Report - Narrowing a region on rat chromosome 13 that protects against hypertension in Dahl SS-13BN congenic strains. - Rat Genome Database

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Narrowing a region on rat chromosome 13 that protects against hypertension in Dahl SS-13BN congenic strains.

Authors: Moreno, Carol  Williams, Jan M  Lu, Limin  Liang, Mingyu  Lazar, Jozef  Jacob, Howard J  Cowley, Allen W  Roman, Richard J 
Citation: Moreno C, etal., Am J Physiol Heart Circ Physiol. 2011 Apr;300(4):H1530-5. doi: 10.1152/ajpheart.01026.2010. Epub 2011 Jan 21.
RGD ID: 12798539
Pubmed: PMID:21257920   (View Abstract at PubMed)
PMCID: PMC3075031   (View Article at PubMed Central)
DOI: DOI:10.1152/ajpheart.01026.2010   (Journal Full-text)

Transfer of chromosome 13 from the Brown Norway (BN) rat onto the Dahl salt-sensitive (SS) genetic background attenuates the development of hypertension, but the genes involved remain to be identified. The purpose of the present study was to confirm by telemetry that a congenic strain [SS.BN-(D13Hmgc37-D13Got22)/Mcwi, line 5], carrying a 13.4-Mb segment of BN chromosome 13 from position 32.4 to 45.8 Mb, is protected from the development of hypertension and then to narrow the region of interest by creating and phenotyping 11 additional subcongenic strains. Mean arterial pressure (MAP) rose from 118 ± 1 to 186 ± 5 mmHg in SS rats fed a high-salt diet (8.0% NaCl) for 3 wk. Protein excretion increased from 56 ± 11 to 365 ± 37 mg/day. In contrast, MAP only increased to 152 ± 9 mmHg in the line 5 congenic strain. Six subcongenic strains carrying segments of BN chromosome 13 from 32.4 and 38.2 Mb and from 39.9 to 45.8 Mb were not protected from the development of hypertension. In contrast, MAP was reduced by ~30 mmHg in five strains, carrying a 1.9-Mb common segment of BN chromosome 13 from 38.5 to 40.4 Mb. Proteinuria was reduced by ~50% in these strains. Sequencing studies did not identify any nonsynonymous single nucleotide polymorphisms in the coding region of the genes in this region. RT-PCR studies indicated that 4 of the 13 genes in this region were differentially expressed in the kidney of two subcongenic strains that were partially protected from hypertension vs. those that were not. These results narrow the region of interest on chromosome 13 from 13.4 Mb (159 genes) to a 1.9-Mb segment containing only 13 genes, of which 4 are differentially expressed in strains partially protected from the development of hypertension.

Disease Annotations    
proteinuria  (IAGP)

Phenotype Annotations    

Mammalian Phenotype

Phenotype Values via PhenoMiner     Click to see Annotation Detail View
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Strains with Phenominer Data Strains with phenominer data
SS/JrHsdMcwi SS-Chr 13BN/Mcwi SS.BN-(D13Rat111-D13Got22)/Mcwi
SS.BN-(D13Hmgc37-D13Got22)/Mcwi SS.BN-(D13Hmgc115-D13Got22)/Mcwi SS.BN-(D13Hmgc86-D13Got22)/Mcwi
SS.BN-(D13Rat20-D13Got22)/Mcwi SS.BN-(D13Rat111-D13Hmgc85)/Mcwi SS.BN-(D13Hmgc40-D13Got22)/Mcwi
SS.BN-(D13Hmgc85-D13Got22)/Mcwi SS.BN-(D13Rat77-D13Got22)/Mcwi SS.BN-(D13Rat111-D13Rat115)/Mcwi
SS.BN-(D13Rat111-D13Hmgc86)/Mcwi SS.BN-(D13Rat111-D13Rat20)/Mcwi

Objects Annotated

Genes (Rattus norvegicus)
Mgat5  (alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase)
Nckap5  (NCK-associated protein 5)
Psme2-ps1  (proteasome activator subunit 2, pseudogene 1)
Tmem163  (transmembrane protein 163)
tuba1c-ps1  (tubulin, alpha 1C, pseudogene 1)

Bp401  (Blood pressure QTL 401)

Additional Information