RGD Reference Report - Narrowing a region on rat chromosome 13 that protects against hypertension in Dahl SS-13BN congenic strains. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Narrowing a region on rat chromosome 13 that protects against hypertension in Dahl SS-13BN congenic strains.

Authors: Moreno, Carol  Williams, Jan M  Lu, Limin  Liang, Mingyu  Lazar, Jozef  Jacob, Howard J  Cowley, Allen W  Roman, Richard J 
Citation: Moreno C, etal., Am J Physiol Heart Circ Physiol. 2011 Apr;300(4):H1530-5. doi: 10.1152/ajpheart.01026.2010. Epub 2011 Jan 21.
RGD ID: 12798539
Pubmed: PMID:21257920   (View Abstract at PubMed)
PMCID: PMC3075031   (View Article at PubMed Central)
DOI: DOI:10.1152/ajpheart.01026.2010   (Journal Full-text)

Transfer of chromosome 13 from the Brown Norway (BN) rat onto the Dahl salt-sensitive (SS) genetic background attenuates the development of hypertension, but the genes involved remain to be identified. The purpose of the present study was to confirm by telemetry that a congenic strain [SS.BN-(D13Hmgc37-D13Got22)/Mcwi, line 5], carrying a 13.4-Mb segment of BN chromosome 13 from position 32.4 to 45.8 Mb, is protected from the development of hypertension and then to narrow the region of interest by creating and phenotyping 11 additional subcongenic strains. Mean arterial pressure (MAP) rose from 118 ± 1 to 186 ± 5 mmHg in SS rats fed a high-salt diet (8.0% NaCl) for 3 wk. Protein excretion increased from 56 ± 11 to 365 ± 37 mg/day. In contrast, MAP only increased to 152 ± 9 mmHg in the line 5 congenic strain. Six subcongenic strains carrying segments of BN chromosome 13 from 32.4 and 38.2 Mb and from 39.9 to 45.8 Mb were not protected from the development of hypertension. In contrast, MAP was reduced by ~30 mmHg in five strains, carrying a 1.9-Mb common segment of BN chromosome 13 from 38.5 to 40.4 Mb. Proteinuria was reduced by ~50% in these strains. Sequencing studies did not identify any nonsynonymous single nucleotide polymorphisms in the coding region of the genes in this region. RT-PCR studies indicated that 4 of the 13 genes in this region were differentially expressed in the kidney of two subcongenic strains that were partially protected from hypertension vs. those that were not. These results narrow the region of interest on chromosome 13 from 13.4 Mb (159 genes) to a 1.9-Mb segment containing only 13 genes, of which 4 are differentially expressed in strains partially protected from the development of hypertension.



RGD Manual Disease Annotations    Click to see Annotation Detail View
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Mgat5Ratproteinuria  IAGP  RGD 
Nckap5Ratproteinuria  IAGP  RGD 
Psme2-ps1Ratproteinuria  IAGP  RGD 
SS.BN-(D13Hmgc40-D13Got22)/McwiRatproteinuria inducedIAGPcontrolled sodium content diet RGD 
SS.BN-(D13Hmgc85-D13Got22)/McwiRatproteinuria inducedIAGPcontrolled sodium content diet RGD 
SS.BN-(D13Rat111-D13Hmgc86)/McwiRatproteinuria inducedIAGPcontrolled sodium content diet RGD 
SS.BN-(D13Rat111-D13Rat115)/McwiRatproteinuria inducedIAGPcontrolled sodium content diet RGD 
SS.BN-(D13Rat111-D13Rat20)/McwiRatproteinuria inducedIAGPcontrolled sodium content diet RGD 
SS.BN-(D13Rat77-D13Got22)/McwiRatproteinuria inducedIAGPcontrolled sodium content diet RGD 
SS/JrHsdMcwiRatproteinuria  IAGP  RGD 
Tmem163Ratproteinuria  IAGP  RGD 
tuba1c-ps1Ratproteinuria  IAGP  RGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SS-Chr 13BN/McwiRatdecreased mean systemic arterial blood pressure inducedIAGPcontrolled sodium content dietcompared to SS/JrHsdMcwiRGD 
SS.BN-(D13Hmgc37-D13Got22)/McwiRatdecreased mean systemic arterial blood pressure inducedIAGPcontrolled sodium content dietcompared to SS/JrHsdMcwiRGD 
SS.BN-(D13Hmgc86-D13Got22)/McwiRatdecreased mean systemic arterial blood pressure inducedIAGPcontrolled sodium content dietcompared to SS/JrHsdMcwiRGD 
SS.BN-(D13Rat111-D13Got22)/McwiRatdecreased mean systemic arterial blood pressure inducedIAGPcontrolled sodium content dietcompared to SS/JrHsdMcwiRGD 
SS.BN-(D13Rat111-D13Hmgc85)/McwiRatdecreased mean systemic arterial blood pressure inducedIAGPcontrolled sodium content dietcompared to SS/JrHsdMcwiRGD 
SS.BN-(D13Rat115-D13Got22)/McwiRatdecreased mean systemic arterial blood pressure inducedIAGPcontrolled sodium content dietcompared to SS/JrHsdMcwiRGD 
SS-Chr 13BN/McwiRatdecreased urine protein level inducedIAGPcontrolled sodium content dietcompared to SS/JrHsdMcwiRGD 
SS.BN-(D13Hmgc37-D13Got22)/McwiRatdecreased urine protein level inducedIAGPcontrolled sodium content dietcompared to SS/JrHsdMcwiRGD 
SS.BN-(D13Hmgc86-D13Got22)/McwiRatdecreased urine protein level inducedIAGPcontrolled sodium content dietcompared to SS/JrHsdMcwiRGD 
SS.BN-(D13Rat111-D13Got22)/McwiRatdecreased urine protein level inducedIAGPcontrolled sodium content dietcompared to SS/JrHsdMcwiRGD 
SS.BN-(D13Rat111-D13Hmgc85)/McwiRatdecreased urine protein level inducedIAGPcontrolled sodium content dietcompared to SS/JrHsdMcwiRGD 
SS.BN-(D13Rat115-D13Got22)/McwiRatdecreased urine protein level inducedIAGPcontrolled sodium content dietcompared to SS/JrHsdMcwiRGD 
SS.BN-(D13Rat77-D13Got22)/McwiRatincreased urine protein level inducedIAGPcontrolled sodium content dietcompared to SS/JrHsdMcwiRGD 
Mgat5Ratsalt-sensitive hypertension  IAGP  RGD 
Nckap5Ratsalt-sensitive hypertension  IAGP  RGD 
Psme2-ps1Ratsalt-sensitive hypertension  IAGP  RGD 
SS.BN-(D13Hmgc40-D13Got22)/McwiRatsalt-sensitive hypertension inducedIAGPcontrolled sodium content diet RGD 
SS.BN-(D13Hmgc85-D13Got22)/McwiRatsalt-sensitive hypertension inducedIAGPcontrolled sodium content diet RGD 
SS.BN-(D13Rat111-D13Hmgc86)/McwiRatsalt-sensitive hypertension inducedIAGPcontrolled sodium content diet RGD 
SS.BN-(D13Rat111-D13Rat115)/McwiRatsalt-sensitive hypertension inducedIAGPcontrolled sodium content diet RGD 
SS.BN-(D13Rat111-D13Rat20)/McwiRatsalt-sensitive hypertension inducedIAGPcontrolled sodium content diet RGD 
SS.BN-(D13Rat77-D13Got22)/McwiRatsalt-sensitive hypertension inducedIAGPcontrolled sodium content diet RGD 
SS/JrHsdMcwiRatsalt-sensitive hypertension  IAGP  RGD 
Tmem163Ratsalt-sensitive hypertension  IAGP  RGD 
tuba1c-ps1Ratsalt-sensitive hypertension  IAGP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mgat5  (alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase)
Nckap5  (NCK-associated protein 5)
Psme2-ps1  (proteasome activator subunit 2, pseudogene 1)
Tmem163  (transmembrane protein 163)
tuba1c-ps1  (tubulin, alpha 1C, pseudogene 1)

QTLs
Bp401  (Blood pressure QTL 401)


Additional Information