RGD Reference Report - Modifications in the biophysical properties of connexin43 channels by a peptide of the cytoplasmic loop region. - Rat Genome Database

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Modifications in the biophysical properties of connexin43 channels by a peptide of the cytoplasmic loop region.

Authors: Seki, Akiko  Duffy, Heather S  Coombs, Wanda  Spray, David C  Taffet, Steven M  Delmar, Mario 
Citation: Seki A, etal., Circ Res. 2004 Aug 20;95(4):e22-8. Epub 2004 Jul 29.
RGD ID: 12793029
Pubmed: PMID:15284189   (View Abstract at PubMed)
DOI: DOI:10.1161/01.RES.0000140737.62245.c5   (Journal Full-text)

Connexin43 (Cx43) channels reside in at least 3 states: closed, open, or residual. It is hypothesized that the residual state results from the interaction of an intracellular "gating element" with structures at the vestibule of the pore. Recently, we showed in vitro that there is an intramolecular interaction of the carboxyl-terminal domain (referred to as "CT") with a region in the cytoplasmic loop of Cx43 (amino acids 119 to 144; referred to as "L2"). Here, we assessed whether the L2 region was able to interact with the gating particle in a functional channel. Cx43 channels were recorded in the presence of a peptide corresponding to the L2 region, delivered via the patch pipette. This manipulation did not modify unitary conductance, but decreased the frequency of transitions into the residual state, prolonged open time, and altered the voltage dependence of the channel in a manner analogous to that observed after truncation of the CT domain. The latter correlated with the ability of the peptide to bind to the CT domain, as determined by mirror resonance spectroscopy. Overall, we propose that the L2 acts as a "receptor" that interacts with a flexible intracellular gating element during channel gating. The full text of this article is available online at http://circres.ahajournals.org.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gja1Ratregulation of transmembrane transporter activity involved_inIMP PMID:15284189CAFA 
Gja1Rattransmembrane transport involved_inIDA PMID:15284189CAFA 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gja1Ratconnexin complex part_ofIDA PMID:15284189CAFA 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gja1Ratgap junction channel activity enablesIDA PMID:15284189CAFA 

Objects Annotated

Genes (Rattus norvegicus)
Gja1  (gap junction protein, alpha 1)


Additional Information