RGD Reference Report - Serum Lipocalin2 is a potential biomarker of liver irradiation damage. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Serum Lipocalin2 is a potential biomarker of liver irradiation damage.

Authors: Sultan, Sadaf  Cameron, Silke  Ahmad, Shakil  Malik, Ihtzaz A  Schultze, Frank C  Hielscher, Ruth  Rave-Fränk, Margret  Hess, Clemens F  Ramadori, Giuliano  Christiansen, Hans 
Citation: Sultan S, etal., Liver Int. 2013 Mar;33(3):459-68. doi: 10.1111/liv.12073. Epub 2013 Jan 18.
RGD ID: 126790570
Pubmed: PMID:23331620   (View Abstract at PubMed)
DOI: DOI:10.1111/liv.12073   (Journal Full-text)


BACKGROUND/AIM: IL-6 - IL-1- lipocalin2 (LCN2) - liver irradiation - oxidative stress - TNF-a Lipocalin2 (LCN2) is an acute phase protein. The source of its increased serum level in oxidative stress conditions (ROS) remains still unknown. We prospectively evaluate the serum LCN2 increase after single dose liver irradiation along with hepatic LCN2 gene and protein expression.
METHODS: A single dose of 25 Gray was administered percutaneously to the liver of randomly paired rats after a planning CT scan. Male Wistar rats were sacrificed 1, 3, 6, 12, 24 and 48 h after irradiation along with sham-irradiated controls. ELISA, RT-PCR, Western blot and immunofluorescence staining was performed. Furthermore, hepatocytes, myofibroblasts and Kupffer cells were isolated from the liver of healthy rats and irradiated ex-vivo.
RESULTS: After liver irradiation, LCN2 serum levels increased significantly up to 2.7 μg/ml within 6 h and stayed elevated over 24 h. LCN2 specific transcripts increased significantly up to 552 ± 109-fold at 24 h after liver irradiation, which was further confirmed at protein level. α2-macroglobulin and hemoxygenase-1 also showed an increase, but the magnitude was less as compared to LCN2. LCN2+ granulocytes were detected within 1 h after irradiation around central and portal fields and remained high during the course of study. Ex-vivo irradiated hepatocytes (2.4 ± 0.6-fold) showed a higher LCN2 gene expression as compared to myofibroblasts and Kupffer cells. IL-1β treatment further increased LCN2 gene expression in cultured hepatocytes.
CONCLUSIONS: Single dose liver irradiation induces a significant increase in LCN2 serum levels, comparable to the induction of acute phase proteins. We suggest LCN2 as marker for the early phase of radiation-induced tissue damage.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
LCN2HumanExperimental Radiation Injuries  ISOLcn2 (Rattus norvegicus)mRNA more ...RGD 
Lcn2RatExperimental Radiation Injuries  IEP mRNA more ...RGD 
Lcn2MouseExperimental Radiation Injuries  ISOLcn2 (Rattus norvegicus)mRNA more ...RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Lcn2Ratcellular response to X-ray  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Lcn2  (lipocalin 2)

Genes (Mus musculus)
Lcn2  (lipocalin 2)

Genes (Homo sapiens)
LCN2  (lipocalin 2)


Additional Information