RGD Reference Report - Role of iron in brain lipocalin 2 upregulation after intracerebral hemorrhage in rats. - Rat Genome Database

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Role of iron in brain lipocalin 2 upregulation after intracerebral hemorrhage in rats.

Authors: Dong, Ming  Xi, Guohua  Keep, Richard F  Hua, Ya 
Citation: Dong M, etal., Brain Res. 2013 Apr 10;1505:86-92. doi: 10.1016/j.brainres.2013.02.008. Epub 2013 Feb 15.
RGD ID: 126790480
Pubmed: PMID:23416150   (View Abstract at PubMed)
PMCID: PMC3605267   (View Article at PubMed Central)
DOI: DOI:10.1016/j.brainres.2013.02.008   (Journal Full-text)

Brain iron overload has a detrimental role in brain injury after intracerebral hemorrhage (ICH). Lipocalin 2 (LCN2), a siderophore-binding protein, is involved in cellular iron transport. The present study investigated changes in LCN2 expression after ICH and the role of iron in those changes. Male Sprague-Dawley rats had an intracaudate injection of autologous blood (ICH) or iron. Control rats received a needle insertion or saline injection. Some ICH animals were treated with either vehicle or deferoxamine, an iron chelator. Brain LCN2 expression was determined by Western blot analysis and immunohistochemistry. Real-time PCR was also used to confirm brain LCN2 mRNA expression. The number of LCN2 positive cells was markedly increased in the ipsilateral basal ganglia and cortex after ICH and most LCN2 positive cells were astrocytes. Western blots showed that brain LCN2 levels were higher at days 1, 3 and 7 in the ipsilateral hemisphere after ICH (70 to 80 fold higher than contralateral hemisphere or sham-operated rats at 3 days), and declined to lower levels at day 14. Iron, but not saline injection also caused brain LCN2 upregulation (a more than 100-fold increase). In addition, systemic treatment of deferoxamine reduced ICH-induced LCN2 upregulation (p<0.05). These results suggest that iron has a role in brain LCN2 upregulation following ICH. LCN2 upregulation after ICH may be part of the response to clear iron released from the hematoma during clot resolution.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
LCN2HumanCerebral Hemorrhage  ISORGD:69408 RGD 
Lcn2RatCerebral Hemorrhage  IEP  RGD 
Lcn2MouseCerebral Hemorrhage  ISORGD:69408 RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Lcn2Ratresponse to iron(II) ion  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Lcn2  (lipocalin 2)

Genes (Mus musculus)
Lcn2  (lipocalin 2)

Genes (Homo sapiens)
LCN2  (lipocalin 2)


Additional Information