RGD Reference Report - Evidence for early endosome-like fusion of recently endocytosed synaptic vesicles. - Rat Genome Database

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Evidence for early endosome-like fusion of recently endocytosed synaptic vesicles.

Authors: Rizzoli, Silvio O  Bethani, Ioanna  Zwilling, Daniel  Wenzel, Dirk  Siddiqui, Tabrez J  Brandhorst, Dorothea  Jahn, Reinhard 
Citation: Rizzoli SO, etal., Traffic. 2006 Sep;7(9):1163-76.
RGD ID: 12050136
Pubmed: PMID:17004320   (View Abstract at PubMed)

Early endosomes are well-established acceptor compartments of endocytic vesicles in many cell types. Little evidence of their existence or function has been obtained in synapses, and it is generally believed that synaptic vesicles recycle without passing through an endosomal intermediate. We show here that the early endosomal SNARE proteins are enriched in synaptic vesicles. To investigate their function in the synapse, we isolated synaptic nerve terminals (synaptosomes), stimulated them in presence of different fluorescent markers to label the recycling vesicles and used these vesicles in in vitro fusion assays. The recently endocytosed vesicles underwent homotypic fusion. They also fused with endosomes from PC12 and BHK cells. The fusion process was dependent upon NSF activity. Moreover, fusion was dependent upon the early endosomal SNAREs but not upon the SNAREs involved in exocytosis. Our results thus show that at least a fraction of the vesicles endocytosed during synaptic activity are capable of fusing with early endosomes and lend support to an involvement of endosomal intermediates during recycling of synaptic vesicles.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Stx12Ratsynaptic vesicle membrane is_active_inIEP PMID:17004320SynGO 
Stx12Ratsynaptic vesicle membrane is_active_inIDA PMID:17004320SynGO 
Stx6Ratsynaptic vesicle membrane is_active_inIDA PMID:17004320SynGO 
Stx6Ratsynaptic vesicle membrane is_active_inIEP PMID:17004320SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Stx12  (syntaxin 12)
Stx6  (syntaxin 6)
Vamp4  (vesicle-associated membrane protein 4)
Vti1a  (vesicle transport through interaction with t-SNAREs 1A)

Objects referenced in this article
Gene Syp synaptophysin Rattus norvegicus

Additional Information