RGD Reference Report - Hypomorphic phenotype of Foxn1 gene-modified rats by CRISPR/Cas9 system. - Rat Genome Database

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Hypomorphic phenotype of Foxn1 gene-modified rats by CRISPR/Cas9 system.

Authors: Goto, T  Hara, H  Nakauchi, H  Hochi, S  Hirabayashi, M 
Citation: Goto T, etal., Transgenic Res. 2016 Aug;25(4):533-44. doi: 10.1007/s11248-016-9941-9. Epub 2016 Mar 2.
RGD ID: 11568681
Pubmed: PMID:26931321   (View Abstract at PubMed)
DOI: DOI:10.1007/s11248-016-9941-9   (Journal Full-text)

The Foxn1 gene is known as a critical factor for the differentiation of thymic and skin epithelial cells. This study was designed to examine the phenotype of Foxn1-modified rats generated by the CRISPR/Cas9 system. Guide-RNA designed for first exon of the Foxn1 and mRNA of Cas9 were co-injected into the pronucleus of Crlj:WI zygotes. Transfer of 158 injected zygotes resulted in the birth of 50 offspring (32 %), and PCR identified five (10 %) as Foxn1-edited. Genomic sequencing revealed the deletion of 44 or 60 bp from and/or insertion of 4 bp into the Foxn1 gene in a single allele. The number of T-cells in the peripheral blood lymphocytes of mutant rats decreased markedly. While homozygous deleted mutant rats had no thymus, the mutant rats were not completely hairless and showed normal performance in delivery and nursing. Splicing variants of the indel-mutation in the Foxn1 gene may cause hypomorphic allele, resulting in the phenotype of thymus deficiency and incomplete hairless. In conclusion, the mutant rats in Foxn1 gene edited by the CRISPR/Cas9 system showed the phenotype of thymus deficiency and incomplete hairless which was characterized by splicing variants.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FOXN1HumanThymus Hyperplasia  ISOFoxn1 (Rattus norvegicus) RGD 
Foxn1RatThymus Hyperplasia  IMP  RGD 
Foxn1MouseThymus Hyperplasia  ISOFoxn1 (Rattus norvegicus) RGD 
Foxn1em1NipsRatThymus Hyperplasia  IMP  RGD 
Foxn1em2NipsRatThymus Hyperplasia  IMP  RGD 
WI-Foxn1em1NipsRatThymus Hyperplasia MODEL:spontaneousIMP  RGD 
WI-Foxn1em1NipsRatThymus Hyperplasia  IMP  RGD 
WI-Foxn1em2NipsRatThymus Hyperplasia MODEL:spontaneousIMP  RGD 
WI-Foxn1em2NipsRatThymus Hyperplasia  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Foxn1  (forkhead box N1)
Foxn1em1Nips  (forkhead box N1; CRISPR/Cas9 system induced mutant 1, National Institute for Physiological Sciences)
Foxn1em2Nips  (forkhead box N1; CRISPR/Cas9 system induced mutant 2, National Institute for Physiological Sciences)

Genes (Mus musculus)
Foxn1  (forkhead box N1)

Genes (Homo sapiens)
FOXN1  (forkhead box N1)

Strains
WI-Foxn1em1Nips  (NA)
WI-Foxn1em2Nips  (NA)


Additional Information