RGD Reference Report - Structure of the voltage-gated K(+) channel Eag1 reveals an alternative voltage sensing mechanism. - Rat Genome Database

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Structure of the voltage-gated K(+) channel Eag1 reveals an alternative voltage sensing mechanism.

Authors: Whicher, JR  Mackinnon, R 
Citation: Whicher JR and MacKinnon R, Science. 2016 Aug 12;353(6300):664-9. doi: 10.1126/science.aaf8070.
RGD ID: 11533419
Pubmed: PMID:27516594   (View Abstract at PubMed)
PMCID: PMC5477842   (View Article at PubMed Central)
DOI: DOI:10.1126/science.aaf8070   (Journal Full-text)

Voltage-gated potassium (K(v)) channels are gated by the movement of the transmembrane voltage sensor, which is coupled, through the helical S4-S5 linker, to the potassium pore. We determined the single-particle cryo-electron microscopy structure of mammalian K(v)10.1, or Eag1, bound to the channel inhibitor calmodulin, at 3.78 angstrom resolution. Unlike previous K(v) structures, the S4-S5 linker of Eag1 is a five-residue loop and the transmembrane segments are not domain swapped, which suggest an alternative mechanism of voltage-dependent gating. Additionally, the structure and position of the S4-S5 linker allow calmodulin to bind to the intracellular domains and to close the potassium pore, independent of voltage-sensor position. The structure reveals an alternative gating mechanism for K(v) channels and provides a template to further understand the gating properties of Eag1 and related channels.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnh1Ratcellular response to calcium ion involved_inIDA PMID:27516594UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnh1Ratplasma membrane located_inIDA PMID:27516594UniProt 
Kcnh1Ratpotassium channel complex part_ofIDA PMID:27516594UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnh1Ratcalmodulin binding enablesIDA PMID:27516594UniProt 
Kcnh1Ratdelayed rectifier potassium channel activity enablesIDA PMID:27516594UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Kcnh1  (potassium voltage-gated channel subfamily H member 1)


Additional Information