RGD Reference Report - Repetitive hyperbaric oxygenation attenuates reactive astrogliosis and suppresses expression of inflammatory mediators in the rat model of brain injury.

Advanced Search (OLGA)

General

Repetitive hyperbaric oxygenation attenuates reactive astrogliosis and suppresses expression of inflammatory mediators in the rat model of brain injury.
Authors:	Lavrnja, I Parabucki, A Brkic, P Jovanovic, T Dacic, S Savic, D Pantic, I Stojiljkovic, M Pekovic, S
Citation:	Lavrnja I, etal., Mediators Inflamm. 2015;2015:498405. doi: 10.1155/2015/498405. Epub 2015 Apr 20.
RGD ID:	11344972
Pubmed:	PMID:25972624 (View Abstract at PubMed)
PMCID:	PMC4417949 (View Article at PubMed Central)
DOI:	DOI:10.1155/2015/498405 (Journal Full-text)
The exact mechanisms by which treatment with hyperbaric oxygen (HBOT) exerts its beneficial effects on recovery after brain injury are still unrevealed. Therefore, in this study we investigated the influence of repetitive HBOT on the reactive astrogliosis and expression of mediators of inflammation after cortical stab injury (CSI). CSI was performed on male Wistar rats, divided into control, sham, and lesioned groups with appropriate HBO. The HBOT protocol was as follows: 10 minutes of slow compression, 2.5 atmospheres absolute (ATA) for 60 minutes, and 10 minutes of slow decompression, once a day for 10 consecutive days. Data obtained using real-time polymerase chain reaction, Western blot, and immunohistochemical and immunofluorescence analyses revealed that repetitive HBOT applied after the CSI attenuates reactive astrogliosis and glial scarring, and reduces expression of GFAP (glial fibrillary acidic protein), vimentin, and ICAM-1 (intercellular adhesion molecule-1) both at gene and tissue levels. In addition, HBOT prevents expression of CD40 and its ligand CD40L on microglia, neutrophils, cortical neurons, and reactive astrocytes. Accordingly, repetitive HBOT, by prevention of glial scarring and limiting of expression of inflammatory mediators, supports formation of more permissive environment for repair and regeneration.

Annotation Click to see Annotation Detail View

RGD Manual Disease Annotations Click to see Annotation Detail View

Only show annotations with direct experimental evidence (0 objects hidden)

Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
CD40LG	Human	Brain Injuries	treatment	ISO	Cd40lg (Rattus norvegicus)		RGD
Cd40lg	Rat	Brain Injuries	treatment	IDA			RGD
Cd40lg	Mouse	Brain Injuries	treatment	ISO	Cd40lg (Rattus norvegicus)		RGD

Gene Ontology Annotations Click to see Annotation Detail View

Cellular Component

Only show annotations with direct experimental evidence (0 objects hidden)

Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
Cd40lg	Rat	cell body		IDA			RGD
Cd40lg	Rat	cell projection		IDA			RGD
Cd40	Rat	neuronal cell body		IDA			RGD
Cd40	Rat	varicosity		IDA			RGD

Objects Annotated

Genes (Rattus norvegicus)

Cd40 (CD40 molecule)

Cd40lg (CD40 ligand)

Genes (Mus musculus)

Cd40lg (CD40 ligand)

Genes (Homo sapiens)

CD40LG (CD40 ligand)

Additional Information

Gene Annotator (Functional Annotation) unavailable	Gene Annotator (Annotation Distribution) unavailable	Variant Visualizer (Genomic Variants) unavailble Variant Visualizer (Genomic Variants) unavailable
Gene Annotator (Functional Annotation)	Gene Annotator (Annotation Distribution)	Variant Visualizer (Genomic Variants)

InterViewer (Protein-Protein Interactions) unavailable	Gviewer (Genome Viewer) unavailable	Variant Visualizer (Damaging Variants) unavailble Variant Visualizer (Damaging Variants) unavailable
InterViewer (Protein-Protein Interactions)	GViewer (Genome Viewer)	Variant Visualizer (Damaging Variants)

Gene Annotator (Annotation Comparison) unavailable	OLGA (Gene List Generator) unavailable
Gene Annotator (Annotation Comparison)	OLGA (Gene List Generator)	Excel (Download)

MOET (Multi-Ontology Enrichement) unavailable	GOLF (Gene-Ortholog Location Finder) unavailable
MOET (Multi-Ontology Enrichement)	GOLF (Gene-Ortholog Location Finder)

Create Name:

Description:

Send us a Message

Repetitive hyperbaric oxygenation attenuates reactive astrogliosis and suppresses expression of inflammatory mediators in the rat model of brain injury.