RGD Reference Report - Crystal structure of the epithelial calcium channel TRPV6. - Rat Genome Database

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Crystal structure of the epithelial calcium channel TRPV6.

Authors: Saotome, K  Singh, AK  Yelshanskaya, MV  Sobolevsky, AI 
Citation: Saotome K, etal., Nature. 2016 Jun 13;534(7608):506-11. doi: 10.1038/nature17975.
RGD ID: 11344952
Pubmed: PMID:27296226   (View Abstract at PubMed)
PMCID: PMC4919205   (View Article at PubMed Central)
DOI: DOI:10.1038/nature17975   (Journal Full-text)

Precise regulation of calcium homeostasis is essential for many physiological functions. The Ca(2+)-selective transient receptor potential (TRP) channels TRPV5 and TRPV6 play vital roles in calcium homeostasis as Ca(2+) uptake channels in epithelial tissues. Detailed structural bases for their assembly and Ca(2+) permeation remain obscure. Here we report the crystal structure of rat TRPV6 at 3.25 A resolution. The overall architecture of TRPV6 reveals shared and unique features compared with other TRP channels. Intracellular domains engage in extensive interactions to form an intracellular 'skirt' involved in allosteric modulation. In the K(+) channel-like transmembrane domain, Ca(2+) selectivity is determined by direct coordination of Ca(2+) by a ring of aspartate side chains in the selectivity filter. On the basis of crystallographically identified cation-binding sites at the pore axis and extracellular vestibule, we propose a Ca(2+) permeation mechanism. Our results provide a structural foundation for understanding the regulation of epithelial Ca(2+) uptake and its role in pathophysiology.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Trpv6Ratcalcium ion transmembrane transport involved_inIDA PMID:27296226UniProt 
Trpv6Ratprotein homotetramerization involved_inIPIUniProtKB:Q9R186PMID:27296226UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Trpv6Ratcalcium channel activity enablesIDA PMID:27296226UniProt 
Trpv6Ratidentical protein binding enablesIPIUniProtKB:Q9R186PMID:27296226IntAct 

Objects Annotated

Genes (Rattus norvegicus)
Trpv6  (transient receptor potential cation channel, subfamily V, member 6)


Additional Information