RGD Reference Report - Experimental priapism is associated with increased oxidative stress and activation of protein degradation pathways in corporal tissue. - Rat Genome Database

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Experimental priapism is associated with increased oxidative stress and activation of protein degradation pathways in corporal tissue.

Authors: Kanika, ND  Melman, A  Davies, KP 
Citation: Kanika ND, etal., Int J Impot Res. 2010 Nov-Dec;22(6):363-73. doi: 10.1038/ijir.2010.27. Epub 2010 Nov 18.
RGD ID: 10412315
Pubmed: PMID:21085184   (View Abstract at PubMed)
PMCID: PMC3058910   (View Article at PubMed Central)
DOI: DOI:10.1038/ijir.2010.27   (Journal Full-text)

Priapism is a debilitating disease for which there is at present no clinically accepted pharmacological intervention. It has been estimated that priapism lasting more than 24 h in patients is associated with a 44-90% rate of ED. In this investigation, we determined in two animal models of priapism (opiorphin-induced priapism in the rat and priapism in a mouse model of sickle cell disease) if there is evidence for an increase in markers of oxidative stress in corporal tissue. In both animal models, we demonstrate that priapism results in increased levels of lipid peroxidation, glutathione S-transferase activity and oxidatively damaged proteins in corporal tissue. Using western blot analysis, we demonstrated there is upregulation of the ubiquitination ligase proteins, Nedd-4 and Mdm-2, and the lysosomal autophage protein, LC3. The antiapoptotic protein, Bcl-2, was also upregulated. Overall, we demonstrate that priapism is associated with increased oxidative stress in corporal tissue and the activation of protein degradation pathways. As oxidative stress is known to mediate the development of ED resulting from several etiologies (for example, ED resulting from diabetes and aging), we suggest that damage to erectile tissue resulting from priapism might be prevented by treatments targeting oxidative stress.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
BCL2Humanpriapism  ISOBcl2 (Rattus norvegicus)protein:increased expression:erectile tissue (rat)RGD 
Bcl2Ratpriapism  IEP protein:increased expression:erectile tissue (rat)RGD 
Bcl2Mousepriapism  ISOBcl2 (Rattus norvegicus)protein:increased expression:erectile tissue (rat)RGD 
MDM2Humanpriapism  ISOMdm2 (Rattus norvegicus)protein:increased expression:corpus cavernosumRGD 
Mdm2Ratpriapism  IEP protein:increased expression:corpus cavernosumRGD 
Mdm2Mousepriapism  ISOMdm2 (Rattus norvegicus)protein:increased expression:corpus cavernosumRGD 
MDM2Humansickle cell anemia  ISOMdm2 (Mus musculus) RGD 
Mdm2Ratsickle cell anemia  ISOMdm2 (Mus musculus) RGD 
Mdm2Mousesickle cell anemia  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Bcl2  (BCL2, apoptosis regulator)
Mdm2  (MDM2 proto-oncogene)

Genes (Mus musculus)
Bcl2  (B cell leukemia/lymphoma 2)
Mdm2  (transformed mouse 3T3 cell double minute 2)

Genes (Homo sapiens)
BCL2  (BCL2 apoptosis regulator)
MDM2  (MDM2 proto-oncogene)


Additional Information