RGD Reference Report - Allosteric Regulation in Gating the Central Channel of the Nuclear Pore Complex. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Allosteric Regulation in Gating the Central Channel of the Nuclear Pore Complex.

Authors: Koh, J  Blobel, G 
Citation: Koh J and Blobel G, Cell. 2015 Jun 4;161(6):1361-73. doi: 10.1016/j.cell.2015.05.013.
RGD ID: 10401147
Pubmed: PMID:26046439   (View Abstract at PubMed)
DOI: DOI:10.1016/j.cell.2015.05.013   (Journal Full-text)

The nuclear pore complex (NPC) arose in evolution as the cell's largest and most versatile transport channel. Current models for selective transport mediated by NPCs are focused on properties of intrinsically disordered regions of nucleoporins that bind transport factors. In contrast, structured regions are considered to provide static anchoring sites for the disordered regions without affecting transport factor binding. Here, we demonstrate allosteric coupling between a structured domain of a channel nucleoporin (Nup58) and its neighboring disordered domain in interaction with another channel nucleoporin (Nup54) and a transport factor (Kapbeta1). Analysis of multiple equilibria showed that multivalent interactions of Kapbeta1 with the disordered domains of Nup58 stabilize the neighboring structured domain associated with Nup54, shifting conformational equilibria from homo-oligomers to hetero-oligomers. Based on these and previous crystallographic results, a quantitative framework was established to describe constriction and dilation of the central channel as a function of transport factor occupancy.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nup54Ratprotein-containing complex  IDA  RGD 
Nup58Ratprotein-containing complex  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nup54Ratprotein binding  IPINup58 (Rattus norvegicus) RGD 
Nup58Ratprotein binding  IPINup54 (Rattus norvegicus) RGD 
Nup54Ratprotein-containing complex binding  IDA heterotetramerization with Nup58RGD 
Nup58Ratprotein-containing complex binding  IDA heterotetramerization with Nup54RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nup54  (nucleoporin 54)
Nup58  (nucleoporin 58)


Additional Information