RGD Reference Report - Differential proteomics analysis of proteins from human diabetic and age-related cataractous lenses. - Rat Genome Database

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Differential proteomics analysis of proteins from human diabetic and age-related cataractous lenses.

Authors: Zhu, J  Shao, J  Yao, Y  Chu, ZD  Yu, QQ  Zhao, W  Lin, Q  Zhang, ZY 
Citation: Zhu J, etal., J Res Med Sci. 2013 Nov;18(11):984-9.
RGD ID: 10059653
Pubmed: PMID:24520233   (View Abstract at PubMed)
PMCID: PMC3906791   (View Article at PubMed Central)

BACKGROUND: To investigate the differential lens proteomics between diabetic cataract, age-related cataract, and natural subjects. MATERIALS AND METHODS: Two-dimensional electrophoresis (2-DE), mass spectrometry (MS), and enzyme-linked immunosorbent assay (ELISA) were employed. Total soluble proteins in lenses of type I diabetic cataract, age-related cataract (nondiabetic) patients, and normal control were extracted and subjected to 2-DE. The differential protein spots were recovered, digested with trypsin, and further applied to MALDI-TOF-MS. ELISA analysis was used to determine the levels of differential proteins in lenses of three groups. RESULTS: 2-DE analysis reflected that lens proteins of normal control, diabetic, and age-related cataract subjects were in the section of pH 5-9 and the relative molecular weights were 14-97 kDa, while relative molecular weight of more abundant crystallines was localized at 20-31 kDa. five differential protein spots were detected and identified using MALDI-TOF-MS, including beta-crystallin A3, alpha-crystallin B chain, chain A of crystal structure of truncated human beta-B1-crystallin, beta-crystallin B1, and an interesting unnamed protein product highly similar to alpha-crystallin B chain, respectively. ELISA analysis revealed that lenses of diabetic cataract patients should contain significantly more concentrations of beta-crystallin A3, alpha-crystallin B chain, and beta-crystallin B1 than those of age-related cataract patients and normal control. CONCLUSION: This study clearly reflected the differential proteins of diabetic cataract, age-related cataract lenses compared with natural subjects, and it is helpful for the further research on the principles and mechanisms of different types of cataract.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CRYBA1Humancataract  IEP associated with Diabetes Mellitus more ...RGD 
Cryba1Ratcataract  ISOCRYBA1 (Homo sapiens)associated with Diabetes Mellitus more ...RGD 
Cryba1Mousecataract  ISOCRYBA1 (Homo sapiens)associated with Diabetes Mellitus more ...RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cryba1  (crystallin, beta A1)

Genes (Mus musculus)
Cryba1  (crystallin, beta A1)

Genes (Homo sapiens)
CRYBA1  (crystallin beta A1)


Additional Information