RGD Reference Report - Lack of pulse and surge modes and glutamatergic stimulation of luteinising hormone release in Kiss1 knockout rats. - Rat Genome Database

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Lack of pulse and surge modes and glutamatergic stimulation of luteinising hormone release in Kiss1 knockout rats.

Authors: Uenoyama, Y  Nakamura, S  Hayakawa, Y  Ikegami, K  Watanabe, Y  Deura, C  Minabe, S  Tomikawa, J  Goto, T  Ieda, N  Inoue, N  Sanbo, M  Tamura, C  Hirabayashi, M  Maeda, KI  Tsukamura, H 
Citation: Uenoyama Y, etal., J Neuroendocrinol. 2015 Mar;27(3):187-97. doi: 10.1111/jne.12257.
RGD ID: 10059342
Pubmed: PMID:25582792   (View Abstract at PubMed)
DOI: DOI:10.1111/jne.12257   (Journal Full-text)

Kisspeptin, encoded by the Kiss1 gene, has attracted attention as a key candidate neuropeptide in controlling puberty and reproduction via regulation of gonadotrophin-releasing hormone (GnRH) secretion in mammals. Pioneer studies with Kiss1 or its cognate receptor Gpr54 knockout (KO) mice showed the indispensable role of kisspeptin-GPR54 signalling in the control of animal reproduction, although detailed analyses of gonadotrophin secretion, especially pulsatile and surge-mode of luteinising hormone (LH) secretion, were limited. Thus, in the present study, we have generated Kiss1 KO rats aiming to evaluate a key role of kisspeptin in governing reproduction via pulse and surge modes of GnRH/LH secretion. Kiss1 KO male and female rats showed a complete suppression of pulsatile LH secretion, which is responsible for folliculogenesis and spermatogenesis, and an absence of puberty and atrophic gonads. Kiss1 KO female rats showed no spontaneous LH/follicle-stimulating hormone surge and an oestrogen-induced LH surge, suggesting that the GnRH surge generation system, which is responsible for ovulation, does not function without kisspeptin. Furthermore, challenge of major stimulatory neurotransmitters, such as monosodium glutamate, NMDA and norepinephrine, failed to stimulate LH secretion in Kiss1 KO rats, albeit they stimulated LH release in wild-type controls. Taken together, the results of the present study confirm that kisspeptin plays an indispensable role in generating two modes (pulse and surge) of GnRH/gonadotrophin secretion to regulate puberty onset and normal reproductive performance. In addition, the present study suggests that kisspeptin neurones play a critical role as a hub integrating major stimulatory neural inputs to GnRH neurones, using newly established Kiss1 KO rats, which serve as a useful model for detailed analysis of hormonal profiles.

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
absent sexual maturation  IMP 10059342; 10059342; 10059342 RGD 
decreased circulating luteinizing hormone level  IMP 10059342; 10059342; 10059342 RGD 
Objects Annotated

Genes (Rattus norvegicus)
Kiss1  (KiSS-1 metastasis-suppressor)
Kiss1tm1Nips  (KiSS-1 metastasis-suppressor; targeted mutant 1, Nips)

Strains
WI;WDB-Kiss1tm1Nips  (NA)


Additional Information