RGD Reference Report - MinK-related peptide 2 modulates Kv2.1 and Kv3.1 potassium channels in mammalian brain. - Rat Genome Database

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MinK-related peptide 2 modulates Kv2.1 and Kv3.1 potassium channels in mammalian brain.

Authors: McCrossan, ZA  Lewis, A  Panaghie, G  Jordan, PN  Christini, DJ  Lerner, DJ  Abbott, GW 
Citation: McCrossan ZA, etal., J Neurosci. 2003 Sep 3;23(22):8077-91.
RGD ID: 10047250
Pubmed: PMID:12954870   (View Abstract at PubMed)
PMCID: PMC6740484   (View Article at PubMed Central)

Delayed rectifier potassium current diversity and regulation are essential for signal processing and integration in neuronal circuits. Here, we investigated a neuronal role for MinK-related peptides (MiRPs), membrane-spanning modulatory subunits that generate phenotypic diversity in cardiac potassium channels. Native coimmunoprecipitation from rat brain membranes identified two novel potassium channel complexes, MiRP2-Kv2.1 and MiRP2-Kv3.1b. MiRP2 reduces the current density of both channels, slows Kv3.1b activation, and slows both activation and deactivation of Kv2.1. Altering native MiRP2 expression levels by RNAi gene silencing or cDNA transfection toggles the magnitude and kinetics of endogenous delayed rectifier currents in PC12 cells and hippocampal neurons. Computer simulations predict that the slower gating of Kv3.1b in complexes with MiRP2 will broaden action potentials and lower sustainable firing frequency. Thus, MiRP2, unlike other known neuronal beta subunits, provides a mechanism for influence over multiple delayed rectifier potassium currents in mammalian CNS via modulation of alpha subunits from structurally and kinetically distinct subfamilies.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnb1Ratdendrite located_inIDA PMID:12954870UniProt 
Kcnb1Ratneuronal cell body membrane located_inIDA PMID:12954870UniProt 
Kcnc1Ratneuronal cell body membrane located_inIDA PMID:12954870UniProt 
Kcnb1Ratperikaryon located_inIDA PMID:12954870UniProt 
Kcne3Ratplasma membrane located_inIDA PMID:12954870UniProt 
Kcnb1Ratvoltage-gated potassium channel complex part_ofIDA PMID:12954870UniProt 
Kcnc1Ratvoltage-gated potassium channel complex part_ofIDA PMID:12954870UniProt 
Kcne3Ratvoltage-gated potassium channel complex located_inIDA PMID:12954870UniProt 

Molecular Function

  

Objects Annotated

Genes (Rattus norvegicus)
Kcnb1  (potassium voltage-gated channel subfamily B member 1)
Kcnc1  (potassium voltage-gated channel subfamily C member 1)
Kcne3  (potassium voltage-gated channel subfamily E regulatory subunit 3)


Additional Information