RGD Reference Report - Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton.

Authors: Zito, K  Knott, G  Shepherd, GM  Shenolikar, S  Svoboda, K 
Citation: Zito K, etal., Neuron. 2004 Oct 14;44(2):321-34.
RGD ID: 10043779
Pubmed: PMID:15473970   (View Abstract at PubMed)
DOI: DOI:10.1016/j.neuron.2004.09.022   (Journal Full-text)

We explored the relationship between regulation of the spine actin cytoskeleton, spine morphogenesis, and synapse formation by manipulating expression of the actin binding protein NrbI and its deletion mutants. In pyramidal neurons of cultured rat hippocampal slices, NrbI is concentrated in dendritic spines by binding to the actin cytoskeleton. Expression of one NrbI deletion mutant, containing the actin binding domain, dramatically increased the density and length of dendritic spines with synapses. This hyperspinogenesis was accompanied by enhanced actin polymerization and spine motility. Synaptic strengths were reduced to compensate for extra synapses, keeping total synaptic input per neuron constant. Our data support a model in which synapse formation is promoted by actin-powered motility.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ppp1r9aRatdendrite  IDA  RGD 
Ppp1r9aRatdendritic spine  IDA  RGD 
Ppp1r9aRatdendritic spine neck  IDA  RGD 
Ppp1r9aRatfilopodium  IDA  RGD 
Ppp1r9aRatglutamatergic synapse is_active_inIDA PMID:15473970SynGO 
Ppp1r9aRatglutamatergic synapse is_active_inIMP PMID:15473970SynGO 
Ppp1r9aRatpostsynaptic actin cytoskeleton is_active_inIDA PMID:15473970SynGO 
Ppp1r9aRatpostsynaptic actin cytoskeleton is_active_inIMP PMID:15473970SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Ppp1r9a  (protein phosphatase 1, regulatory subunit 9A)


Additional Information