Cyp4a8 (cytochrome P450, family 4, subfamily a, polypeptide 8) - Rat Genome Database

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Gene: Cyp4a8 (cytochrome P450, family 4, subfamily a, polypeptide 8) Rattus norvegicus
Analyze
No known orthologs.
Symbol: Cyp4a8
Name: cytochrome P450, family 4, subfamily a, polypeptide 8
RGD ID: 628846
Description: Enables fatty acid omega-hydroxylase activity. Involved in fatty acid metabolic process; kidney development; and response to testosterone. Located in endoplasmic reticulum membrane. Biomarker of autosomal recessive polycystic kidney disease and hypertension. Human ortholog(s) of this gene implicated in hypertension. Orthologous to human CYP4A11 (cytochrome P450 family 4 subfamily A member 11) and CYP4A22 (cytochrome P450 family 4 subfamily A member 22); PARTICIPATES IN arachidonic acid metabolic pathway; eicosanoid signaling pathway via peroxisome proliferator-activated receptor gamma; fatty acid metabolic pathway; INTERACTS WITH 17beta-estradiol; 2,2',4,4'-Tetrabromodiphenyl ether; 2,3,7,8-tetrachlorodibenzodioxine.
Type: protein-coding
RefSeq Status: PROVISIONAL
Previously known as: Cyp4a12; CYPIVA12; CYPIVA8; cytochrome P450 4A12; cytochrome P450, 4a12; cytochrome P450-KP1; cytochrome P450-PP1; MGC93354; P450-KP1; P450-PP1
RGD Orthologs
Alliance Genes
More Info homologs ...
Related Pseudogenes: Cyp4a8-ps1  
Candidate Gene For: Rf54
Latest Assembly: mRatBN7.2 - mRatBN7.2 Assembly
Position:
Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCr85133,938,882 - 133,970,226 (-)NCBIGRCr8
mRatBN7.25128,702,130 - 128,733,476 (-)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 Ensembl5128,702,131 - 128,733,476 (-)EnsemblmRatBN7.2 Ensembl
UTH_Rnor_SHR_Utx5131,384,778 - 131,406,871 (-)NCBIRnor_SHRUTH_Rnor_SHR_Utx
UTH_Rnor_SHRSP_BbbUtx_1.05133,114,432 - 133,136,521 (-)NCBIRnor_SHRSPUTH_Rnor_SHRSP_BbbUtx_1.0
UTH_Rnor_WKY_Bbb_1.05133,136,126 - 133,158,217 (-)NCBIRnor_WKYUTH_Rnor_WKY_Bbb_1.0
Rnor_6.05133,978,953 - 134,008,255 (-)NCBIRnor6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl5133,978,954 - 134,008,255 (-)EnsemblRnor6.0rn6Rnor6.0
Rnor_5.05137,769,865 - 137,799,167 (-)NCBIRnor5.0Rnor_5.0rn5Rnor5.0
RGSC_v3.45135,546,858 - 135,578,199 (-)NCBIRGSC3.4RGSC_v3.4rn4RGSC3.4
RGSC_v3.15135,552,083 - 135,583,425 (-)NCBI
Celera5127,340,416 - 127,371,735 (-)NCBICelera
Cytogenetic Map5q35NCBI
JBrowse: View Region in Genome Browser (JBrowse)
Model


Disease Annotations     Click to see Annotation Detail View

Gene Ontology Annotations     Click to see Annotation Detail View

Cellular Component

References

References - curated
# Reference Title Reference Citation
1. The CYP4A isoforms hydroxylate epoxyeicosatrienoic acids to form high affinity peroxisome proliferator-activated receptor ligands. Cowart LA, etal., J Biol Chem. 2002 Sep 20;277(38):35105-12. Epub 2002 Jul 17.
2. Elevated production of 20-HETE in the cerebral vasculature contributes to severity of ischemic stroke and oxidative stress in spontaneously hypertensive rats. Dunn KM, etal., Am J Physiol Heart Circ Physiol. 2008 Dec;295(6):H2455-65. Epub 2008 Oct 24.
3. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Gaudet P, etal., Brief Bioinform. 2011 Sep;12(5):449-62. doi: 10.1093/bib/bbr042. Epub 2011 Aug 27.
4. Rat ISS GO annotations from GOA human gene data--August 2006 GOA data from the GO Consortium
5. Covalently linked heme in cytochrome p4504a fatty acid hydroxylases. Hoch U and Ortiz De Montellano PR, J Biol Chem. 2001 Apr 6;276(14):11339-46. Epub 2001 Jan 3.
6. Developmentally regulated expression of the CYP4A genes in the spontaneously hypertensive rat kidney. Kroetz DL, etal., Mol Pharmacol. 1997 Sep;52(3):362-72.
7. Kinetic profile of the rat CYP4A isoforms: arachidonic acid metabolism and isoform-specific inhibitors. Nguyen X, etal., Am J Physiol. 1999 Jun;276(6 Pt 2):R1691-700.
8. Chronic blockade of 20-HETE synthesis reduces polycystic kidney disease in an orthologous rat model of ARPKD. Park F, etal., Am J Physiol Renal Physiol. 2009 Jan 7.
9. KEGG Annotation Import Pipeline Pipeline to import KEGG annotations from KEGG into RGD
10. GOA pipeline RGD automated data pipeline
11. Data Import for Chemical-Gene Interactions RGD automated import pipeline for gene-chemical interactions
12. Renal vascular cytochrome P450-derived eicosanoids in androgen-induced hypertension. Singh H and Schwartzman ML, Pharmacol Rep. 2008 Jan-Feb;60(1):29-37.
13. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, etal., Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16899-903. Epub 2002 Dec 11.
14. Cloning and characterization of a novel member of the cytochrome P450 subfamily IVA in rat prostate. Stromstedt M, etal., DNA Cell Biol 1990 Oct;9(8):569-77.
15. Contribution of CYP4A8 to the formation of 20-hydroxyeicosatetraenoic acid from arachidonic acid in rat kidney. Yamaguchi Y, etal., Drug Metab Pharmacokinet. 2002;17(2):109-16.
16. Induction of renal 20-hydroxyeicosatetraenoic acid by clofibrate attenuates high-fat diet-induced hypertension in rats. Zhou Y, etal., J Pharmacol Exp Ther. 2006 Apr;317(1):11-8. Epub 2005 Dec 8.
Additional References at PubMed
PMID:15489334   PMID:25865156  


Genomics

Variants

.
Variants in Cyp4a8
783 total Variants
miRNA Target Status

Predicted Target Of
Summary Value
Count of predictions:94
Count of miRNA genes:78
Interacting mature miRNAs:81
Transcripts:ENSRNOT00000012448
Prediction methods:Miranda, Rnahybrid
Result types:miRGate_prediction

The detailed report is available here: Full Report CSV TAB Printer

miRNA Target Status data imported from miRGate (http://mirgate.bioinfo.cnio.es/).
For more information about miRGate, see PMID:25858286 or access the full paper here.


QTLs in Region (mRatBN7.2)
The following QTLs overlap with this region.    Full Report CSV TAB Printer Gviewer
RGD IDSymbolNameLODP ValueTraitSub TraitChrStartStopSpecies
70212Niddm25Non-insulin dependent diabetes mellitus QTL 253.54blood glucose amount (VT:0000188)blood glucose level (CMO:0000046)51131345958Rat
1576314Eutr1Estrogen induced uterine response QTL 1uterus integrity trait (VT:0010575)pyometritis severity score (CMO:0002009)52138965166875058Rat
2290448Scl54Serum cholesterol level QTL 542.93blood cholesterol amount (VT:0000180)plasma total cholesterol level (CMO:0000585)531663789131345958Rat
1641912Alcrsp18Alcohol response QTL 18response to alcohol trait (VT:0010489)duration of loss of righting reflex (CMO:0002289)535189153141643988Rat
1549845Scl44Serum cholesterol level QTL 446blood cholesterol amount (VT:0000180)serum total cholesterol level (CMO:0000363)540128307148607290Rat
1331801Rf33Renal function QTL 334.149kidney blood vessel physiology trait (VT:0100012)absolute change in renal vascular resistance (CMO:0001900)543726656129132602Rat
1578766Tcas11Tongue tumor susceptibility QTL 114.12tongue integrity trait (VT:0010553)number of squamous cell tumors of the tongue with diameter greater than 3 mm (CMO:0001950)546711509161317411Rat
1576312Emca8Estrogen-induced mammary cancer QTL 84.1mammary gland integrity trait (VT:0010552)mammary tumor number (CMO:0000343)550328551141643988Rat
70189Mcs5Mammary carcinoma susceptibility QTL 510.51mammary gland integrity trait (VT:0010552)mammary tumor number (CMO:0000343)555805606132207589Rat
61426Scl2Serum cholesterol level QTL 27.30.001blood cholesterol amount (VT:0000180)serum total cholesterol level (CMO:0000363)559793399143070159Rat
61393Bp7Blood pressure QTL 74.50.0001arterial blood pressure trait (VT:2000000)systolic blood pressure (CMO:0000004)560293434161481680Rat
1354598Srn6Serum renin concentration QTL 63.8blood renin amount (VT:0003349)plasma renin activity level (CMO:0000116)569540295151018848Rat
1302790Scl20Serum cholesterol level QTL 206.40.0001blood cholesterol amount (VT:0000180)plasma total cholesterol level (CMO:0000585)582394392166664054Rat
1298086Bp156Blood pressure QTL 156arterial blood pressure trait (VT:2000000)systolic blood pressure (CMO:0000004)584132602129132602Rat
7411601Foco12Food consumption QTL 1219.70.001eating behavior trait (VT:0001431)feed conversion ratio (CMO:0001312)587468046132468046Rat
7411564Bw135Body weight QTL 1350.001body mass (VT:0001259)body weight gain (CMO:0000420)587468046132468046Rat
7411582Foco3Food consumption QTL 37.50.001eating behavior trait (VT:0001431)feed conversion ratio (CMO:0001312)587468046132468046Rat
6903316Bw113Body weight QTL 11320.0103body mass (VT:0001259)body weight (CMO:0000012)587765973132765973Rat
631527Tls1T-lymphoma susceptibility QTL 100.001thymus integrity trait (VT:0010555)post-insult time to onset of T-cell lymphoma (CMO:0001907)590450144135450144Rat
61452Ciaa5CIA Autoantibody QTL 53.5blood autoantibody amount (VT:0003725)calculated serum anti-rat type 2 collagen autoantibody titer (CMO:0001281)594858972143070159Rat
1331796Thshl2Thyroid stimulating hormone level QTL 22.3blood thyroid-stimulating hormone amount (VT:0005119)serum thyroid stimulating hormone level (CMO:0001248)597059760147465714Rat
2317753Glom24Glomerulus QTL 243.1kidney glomerulus integrity trait (VT:0010546)kidney sclerotic glomeruli count to total glomeruli count ratio (CMO:0001269)597570330136479578Rat
1358187Emca1Estrogen-induced mammary cancer QTL 14.4mammary gland integrity trait (VT:0010552)post-insult time to mammary tumor formation (CMO:0000345)599216724148607142Rat
1578673Bmd13Bone mineral density QTL 134.9femur mineral mass (VT:0010011)trabecular volumetric bone mineral density (CMO:0001729)5103689353148689353Rat
2317056Wbc3White blood cell count QTL 32.510.01leukocyte quantity (VT:0000217)white blood cell count (CMO:0000027)5105999803150999803Rat
7207488Bss110Bone structure and strength QTL 18.4femur strength trait (VT:0010010)femur stiffness (CMO:0001674)5106906205151906205Rat
7207491Bss112Bone structure and strength QTL 1127femur morphology trait (VT:0000559)femur midshaft cortical cross-sectional area (CMO:0001663)5106906205151906205Rat
7207481Bss106Bone structure and strength QTL 1067.9femur strength trait (VT:0010010)femur ultimate force (CMO:0001675)5106906205151906205Rat
7207486Bss109Bone structure and strength QTL 109femur strength trait (VT:0010010)femur total energy absorbed before break (CMO:0001677)5106906205151906205Rat
1549838Bss4Bone structure and strength QTL 49.2femur strength trait (VT:0010010)femur midshaft polar moment of inertia (CMO:0001669)5106906205151906205Rat
1298089Scl14Serum cholesterol level QTL 145.80.0004blood cholesterol amount (VT:0000180)plasma total cholesterol level (CMO:0000585)5108845856153845856Rat
1598847Cm62Cardiac mass QTL 623.4heart mass (VT:0007028)heart weight to body weight ratio (CMO:0000074)5108845856153845856Rat
8657050Bw146Body weight QTL 14619.840.001body mass (VT:0001259)body weight gain (CMO:0000420)5108938288153938288Rat
8694441Bw169Body weight QTL 16917.610.001retroperitoneal fat pad mass (VT:0010430)retroperitoneal fat pad weight to body weight ratio (CMO:0000635)5111416838156416838Rat
8694198Abfw3Abdominal fat weight QTL 316.130.001visceral adipose mass (VT:0010063)abdominal fat pad weight to body weight ratio (CMO:0000095)5111416838156416838Rat
8694389Bw160Body weight QTL 1606.170.001body lean mass (VT:0010483)lean tissue morphological measurement (CMO:0002184)5111416838156416838Rat
8552960Pigfal15Plasma insulin-like growth factor 1 level QTL 15blood insulin-like growth factor amount (VT:0010479)plasma insulin-like growth factor 1 level (CMO:0001299)5111416838156416838Rat
1581510Cm54Cardiac mass QTL 543.40.05heart left ventricle mass (VT:0007031)heart left ventricle weight to body weight ratio (CMO:0000530)5120740824143608494Rat
2293642Bss37Bone structure and strength QTL 374.640.0001femur strength trait (VT:0010010)femur ultimate force (CMO:0001675)5120740824151018848Rat
1641920Colcs1Colorectal carcinoma susceptibility QTL 12.990.0055intestine integrity trait (VT:0010554)benign colorectal tumor surface area measurement (CMO:0001799)5121846814166846814Rat
7394710Emca12Estrogen-induced mammary cancer QTL 12mammary gland integrity trait (VT:0010552)mammary tumor number (CMO:0000343)5124160767133749643Rat
10053720Scort26Serum corticosterone level QTL 262.060.0147blood corticosterone amount (VT:0005345)plasma corticosterone level (CMO:0001173)5124965598166875058Rat
1300122Wbc1White blood cell count QTL 12.75leukocyte quantity (VT:0000217)total white blood cell count (CMO:0000365)5125392826139989768Rat
724525Bp147Blood pressure QTL 1474.30.0001arterial blood pressure trait (VT:2000000)systolic blood pressure (CMO:0000004)5126424772166875058Rat
1598819Bp292Blood pressure QTL 2924.3arterial blood pressure trait (VT:2000000)systolic blood pressure (CMO:0000004)5127798274166875058Rat
1598861Cm64Cardiac mass QTL 642.9heart mass (VT:0007028)heart weight to body weight ratio (CMO:0000074)5127798274166875058Rat
1581505Rf54Renal function QTL 54kidney physiology trait (VT:0002136)kidney 20-HETE level (CMO:0001854)5128033842133011550Rat
8552908Pigfal4Plasma insulin-like growth factor 1 level QTL 46.6blood insulin-like growth factor amount (VT:0010479)plasma insulin-like growth factor 1 level (CMO:0001299)5128506074166875058Rat
8694169Bw148Body weight QTL 14850.001body mass (VT:0001259)body weight gain (CMO:0000420)5128506074166875058Rat

Markers in Region
RH135045  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.25128,702,633 - 128,702,814 (+)MAPPERmRatBN7.2
Rnor_6.05133,979,457 - 133,979,637NCBIRnor6.0
Rnor_5.05137,770,369 - 137,770,549UniSTSRnor5.0
RGSC_v3.45135,547,362 - 135,547,542UniSTSRGSC3.4
Celera5127,340,920 - 127,341,100UniSTS
RH 3.4 Map5828.6UniSTS
Cytogenetic Map5q36UniSTS
AA893011  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.25128,702,186 - 128,702,420 (+)MAPPERmRatBN7.2
Rnor_6.05133,979,010 - 133,979,243NCBIRnor6.0
Rnor_5.05137,769,922 - 137,770,155UniSTSRnor5.0
RGSC_v3.45135,546,915 - 135,547,148UniSTSRGSC3.4
Celera5127,340,473 - 127,340,706UniSTS
RH 3.4 Map5836.0UniSTS
Cytogenetic Map5q36UniSTS


Expression


RNA-SEQ Expression
High: > 1000 TPM value   Medium: Between 11 and 1000 TPM
Low: Between 0.5 and 10 TPM   Below Cutoff: < 0.5 TPM

alimentary part of gastrointestinal system circulatory system endocrine system exocrine system hemolymphoid system hepatobiliary system integumental system musculoskeletal system nervous system renal system reproductive system respiratory system appendage
High
Medium 7 7 7 13
Low 2 10 31 15 18 15 8 9 9 16 9 10 8
Below cutoff 2 7 7 1 7 1 2 4 11

Sequence


RefSeq Acc Id: ENSRNOT00000012448   ⟹   ENSRNOP00000012448
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
mRatBN7.2 Ensembl5128,702,131 - 128,733,476 (-)Ensembl
Rnor_6.0 Ensembl5133,978,954 - 134,008,255 (-)Ensembl
RefSeq Acc Id: NM_031605   ⟹   NP_113793
RefSeq Status: PROVISIONAL
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr85133,938,882 - 133,970,226 (-)NCBI
mRatBN7.25128,702,130 - 128,733,476 (-)NCBI
Rnor_6.05133,978,953 - 134,008,255 (-)NCBI
Rnor_5.05137,769,865 - 137,799,167 (-)NCBI
RGSC_v3.45135,546,858 - 135,578,199 (-)RGD
Celera5127,340,416 - 127,371,735 (-)RGD
Sequence:
Protein Sequences
Protein RefSeqs NP_113793 (Get FASTA)   NCBI Sequence Viewer  
GenBank Protein AAA63485 (Get FASTA)   NCBI Sequence Viewer  
  AAH81771 (Get FASTA)   NCBI Sequence Viewer  
Ensembl Protein ENSRNOP00000012448
  ENSRNOP00000012448.4
GenBank Protein P24464 (Get FASTA)   NCBI Sequence Viewer  
RefSeq Acc Id: NP_113793   ⟸   NM_031605
- UniProtKB: Q642E9 (UniProtKB/Swiss-Prot),   P24464 (UniProtKB/Swiss-Prot)
- Sequence:
RefSeq Acc Id: ENSRNOP00000012448   ⟸   ENSRNOT00000012448

Protein Structures
Name Modeler Protein Id AA Range Protein Structure
AF-P24464-F1-model_v2 AlphaFold P24464 1-508 view protein structure

Transcriptome

eQTL   View at Phenogen
WGCNA   View at Phenogen
Tissue/Strain Expression   View at Phenogen

Promoters
RGD ID:13693916
Promoter ID:EPDNEW_R4441
Type:multiple initiation site
Name:Cyp4a8_1
Description:cytochrome P450, family 4, subfamily a, polypeptide 8
SO ACC ID:SO:0000170
Source:EPDNEW (Eukaryotic Promoter Database, http://epd.vital-it.ch/)
Experiment Methods:Single-end sequencing.
Position:
Rat AssemblyChrPosition (strand)Source
Rnor_6.05134,008,268 - 134,008,328EPDNEW

Additional Information

Database Acc Id Source(s)
AGR Gene RGD:628846 AgrOrtholog
BioCyc Gene G2FUF-40296 BioCyc
Ensembl Genes ENSRNOG00000008842 Ensembl, ENTREZGENE, UniProtKB/Swiss-Prot
Ensembl Transcript ENSRNOT00000012448 ENTREZGENE
  ENSRNOT00000012448.6 UniProtKB/Swiss-Prot
Gene3D-CATH 1.10.630.10 UniProtKB/Swiss-Prot
IMAGE_CLONE IMAGE:7108258 IMAGE-MGC_LOAD
InterPro Cyt_P450 UniProtKB/Swiss-Prot
  Cyt_P450_CS UniProtKB/Swiss-Prot
  Cyt_P450_E_grp-I UniProtKB/Swiss-Prot
  Cyt_P450_sf UniProtKB/Swiss-Prot
KEGG Report rno:266674 UniProtKB/Swiss-Prot
MGC_CLONE MGC:93354 IMAGE-MGC_LOAD
NCBI Gene 266674 ENTREZGENE
PANTHER CYTOCHROME P450 4A11-RELATED UniProtKB/Swiss-Prot
  CYTOCHROME P450 FAMILY 4 UniProtKB/Swiss-Prot
Pfam p450 UniProtKB/Swiss-Prot
PhenoGen Cyp4a8 PhenoGen
PRINTS EP450I UniProtKB/Swiss-Prot
  P450 UniProtKB/Swiss-Prot
PROSITE CYTOCHROME_P450 UniProtKB/Swiss-Prot
RatGTEx ENSRNOG00000008842 RatGTEx
Superfamily-SCOP SSF48264 UniProtKB/Swiss-Prot
UniProt CP4AC_RAT UniProtKB/Swiss-Prot, ENTREZGENE
  Q642E9 ENTREZGENE
UniProt Secondary Q642E9 UniProtKB/Swiss-Prot


Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2007-07-06 Cyp4a8  cytochrome P450, family 4, subfamily a, polypeptide 8  Cyp4a12  cytochrome P450, 4a12  Name updated 1299863 APPROVED
2003-02-27 Cyp4a12  cytochrome P450, 4a12      Symbol and Name status set to provisional 70820 PROVISIONAL

RGD Curation Notes
Note Type Note Reference
gene_regulation expression is induced by androgen treatment of castrated rats 70705