Defb23<sup>em2Mlit</sup>Defb26<sup>em2Mlit</sup> (defensin beta 23, defensin beta 26; CRISPR/Cas9 induced mutant2, Mlit) - Rat Genome Database

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Gene: Defb23em2MlitDefb26em2Mlit (defensin beta 23, defensin beta 26; CRISPR/Cas9 induced mutant2, Mlit) Rattus norvegicus
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Symbol: Defb23em2MlitDefb26em2Mlit
Name: defensin beta 23, defensin beta 26; CRISPR/Cas9 induced mutant2, Mlit
RGD ID: 38599014
Description: CRISPR/Cas9 system was used to generate this double-gene knockout. The Slc:SD zygotes were injected with sgRNAs that containing 4 sgRNAs (5 ng/ml each) targeting 2 adjacent sites (target sites 1 and 2) of each of the 2 genes. The resulting mutations are 317 bp (Defb23) and 380 bp (Defb26) fragments which were deleted in the Defb23/Defb26 double-mutant strain.
ASSOCIATED WITH decreased litter size; decreased sperm progressive motility; impaired sperm capacitation
Type: allele
Is Marker For: Strains:   SD-Defb23em2MlitDefb26em2Mlit   SD-Defb23em2MlitDefb26em2MlitDefb42em1Mlit  
Latest Assembly: mRatBN7.2 - mRatBN7.2 Assembly
Position: No map positions available.


Phenotype Annotations     Click to see Annotation Detail View

Mammalian Phenotype
References

References - curated
# Reference Title Reference Citation
1. CRISPR/Cas9-mediated genome editing reveals the synergistic effects of ß-defensin family members on sperm maturation in rat epididymis. Zhang C, etal., FASEB J. 2018 Mar;32(3):1354-1363. doi: 10.1096/fj.201700936R. Epub 2018 Jan 3.

Genomics


Related Rat Strains
The following Strains have been annotated to Defb23em2MlitDefb26em2Mlit


Expression


Sequence

Nucleotide Sequences


Additional Information