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Cell Line : CVCL_0023 (A-549) Homo sapiens

Symbol: CVCL_0023
Name: A-549
Cell Line Type: cancer cell line
Gender: Male
Research Use: Omics: Acetylation analysis by proteomics; Omics: Array-based CGH; Omics: CNV analysis; Omics: Deep antibody staining analysis; Omics: Deep exome analysis; Omics: Deep membrane proteome analysis; Omics: Deep phosphoproteome analysis; Omics: Deep proteome analysis; Omics: Deep quantitative proteome analysis; Omics: Deep RNAseq analysis; Omics: DNA methylation analysis; Omics: Fluorescence phenotype profiling; Omics: H3K4me3 ChIP-seq epigenome analysis; Omics: H3K9ac ChIP-seq epigenome analysis; Omics: lncRNA expression profiling; Omics: Metabolome analysis; Omics: Protein expression by reverse-phase protein arrays; Omics: Proteome analysis by 2D-DE/MS; Omics: shRNA library screening; Omics: SNP array analysis; Omics: Transcriptome analysis; Omics: Virome analysis using proteomics
Availability: Discontinued: ATCC; CRL-7909; true.
Phenotype: Doubling time: 18 hours (in RPMI 1640 + 10% FBS), 37 hours (in ACL-3), 36 hours (in ACL-3+BSA) (PubMed=3940644); 27.0 hours (PubMed=8286010); 22 hours (PubMed=25984343); 27 hours (from cell counting), 27 hours (from absorbance) (DOI=10.5897/IJBMBR2013.0154); 22.9 hours (NCI-DTP); ~28 hours (CLS); ~40 hours (DSMZ); Microsatellite instability: Stable (MSS) (PubMed=12661003; Sanger)
Genomic Alteration: Sequence variation: Homozygous for KRAS p.Gly12Ser (c.34G>A) (ClinVar=VCV000012584) (PubMed=12794755; PubMed=17088437); Sequence variation: Homozygous for STK11 p.Gln37Ter (c.109C>T) (ClinVar=VCV000376334) (PubMed=17088437); Sequence variation: Has no TP53 mutation (PubMed=15900046)
Groups: Part of: Cancer Cell Line Encyclopedia (CCLE) project; Part of: COSMIC cell lines project; Part of: ENCODE project common cell types; tier 2; Part of: JFCR39 cancer cell line panel; Part of: KuDOS 95 cell line panel; Part of: MD Anderson Cell Lines Project; Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982); Part of: NCI-60 cancer cell line panel; Part of: NCI-7 clinical proteomics reference material cell line panel
Description: HLA typing: A*25:01,30:01; B*18,44:03:01; C*12:03:01,16:01; DQB1*02:02,03:01:01; DRB1*07:01,11:04:01 (PubMed=15748285); HLA typing: A*25:01,30:01; B*44:03,18:01; C*12:03,16:01; DQB1*02:02,02:02 (PubMed=25960936); HLA typing: A*26:03,30:01; B*18:01,44:03:01; C*12:03,16:01 (PubMed=9023415); Genome ancestry: African=0%; Native American=0%; East Asian, North=0.72%; East Asian, South=0%; South Asian=0%; European, North=66.12%; European, South=33.16% (PubMed=30894373)
Child Cell Lines: CVCL_4V06 (A549(VM)28)
CVCL_4V07 (A549(VP)28)
CVCL_4Z15 (A549.EpoB40)
CVCL_5992 (JHU-028)
CVCL_5I73 (A549-Dual)
CVCL_5I86 (A549-Red-FLuc)
CVCL_5J13 (A549-luc-C8)
CVCL_5J44 (p53-RE-luc/A549)
CVCL_8470 (AE1201)
CVCL_DF39 (A549 NucLight Green)
CVCL_DF40 (A549 NucLight Red)
CVCL_H268 (A549/CPT)
CVCL_IP03 (A549-CR)
CVCL_IY87 (A549/8)
CVCL_J242 (A549-Luc)
CVCL_JY87 (A549/GFP)
CVCL_KS36 (A549/NFkB-luc)
CVCL_KV97 (InCELL Hunter A549 EP300 Bromodomain)
CVCL_KV98 (InCELL Hunter A549 G9a Methyltransferase)
CVCL_KV99 (InCELL Hunter A549 GLP Methyltransferase)
CVCL_KW00 (InCELL Hunter A549 PRMT3 Methyltransferase)
CVCL_KW16 (PathHunter A549 IkappaB Degradation)
CVCL_LB65 (A549 MX10)
CVCL_QZ76 (A549-eGFP-Puro)
CVCL_QZ77 (A549-Fluc-Neo/eGFP-Puro)
CVCL_QZ78 (A549-Fluc-Neo/iRFP-Puro)
CVCL_QZ79 (A549-Fluc-Puro)
CVCL_QZ80 (A549-hNIS-Neo)
CVCL_QZ81 (A549-hNIS-Neo/eGFP-Puro)
CVCL_QZ82 (A549-hNIS-Neo/Fluc-Puro)
CVCL_QZ83 (A549-hNIS-Neo/iRFP-Puro)
CVCL_QZ84 (A549-iRFP-Neo)
CVCL_QZ85 (A549-iRFP-Puro)
CVCL_RQ47 (A549rCDDP2000)
CVCL_RX08 (A549 L2A6)
CVCL_RX09 (A549 L2G9)
CVCL_TZ89 (SL0003)
CVCL_TZ90 (SL0006)
CVCL_U656 (AE1-2a)
CVCL_UJ49 (A549/SF)
CVCL_UR31 (A549-Luc2)
CVCL_VR73 (A549 dCas9-KRAB)
CVCL_W190 (A549/TXL10)
CVCL_W191 (A549/TXL20)
CVCL_W192 (A549/TXL5)
CVCL_W218 (A549-Taxol)
CVCL_W340 (K209)
CVCL_XB27 (A549-tdT)
CVCL_XB30 (A549-mCherry)
CVCL_XE67 (A549-5Fu)
CVCL_XE68 (A549-Cas9-538)
CVCL_XE69 (A549-Luc2-tdT-2)
CVCL_XI06 (A549-ESM)
CVCL_XY99 (A549 OS8)
CVCL_YA87 (A549/Asc-1)
CVCL_YZ46 (A549 DHODH-/-)
CVCL_ZI99 (LanthaScreen ATF2 (19-106) A549)
Aliases: A 549; A549; A549 ATCC; A549/ATCC; A549ATCC; A59; A594; hA549; NCI-A549
Disease(s): lung adenocarcinoma (DOID:3910) [NCI:C3512]


References - uncurated

Additional Information

External Database Links
RGD Object Information
RGD ID: 21113381
Created: 2020-02-27
Species: Homo sapiens
Last Modified: 2020-02-27
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.