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MEASUREMENT METHODS - ANNOTATIONS

The Clinical Measurement Ontology (CMO), Measurement Methods Ontology (MMO), and Experimental Condition Ontology (XCO) are currently being developed at the Rat Genome Database. For more information about these vocabularies please see Shimoyama et al. Three ontologies to define phenotype measurement data. Front Genet. 2012;3:87. Epub 2012 May 28 or contact us (http://rgd.mcw.edu/contact/index.shtml).
Term:polymerase chain reaction assay
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Accession:MMO:0000459 term browser browse the term
Definition:A rapid technique for in vitro amplification of specific DNA sequences, allowing small quantities of short sequences to be analyzed without cloning. DNA sequences are amplified in vitro by separating the DNA into two strands and incubating it in a series of temperature cycles with oligonucleotide primers and heat-stable DNA polymerase.
Synonyms:exact_synonym: PCR


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real-time polymerase chain reaction assay term browser
Symbol Object Name Evidence Notes Source PubMed Reference(s) RGD Reference(s) Position
Q Neuinf10 Neuroinflammation QTL 10 IDA RGD PMID:19525015 RGD:2311085 NCBI chr11:31,239,134...78,851,519 JBrowse link
Q Neuinf11 Neuroinflammation QTL 11 IDA RGD PMID:19525015 RGD:2311085 NCBI chr 3:15,005,422...76,927,699 JBrowse link
Q Neuinf12 Neuroinflammation QTL 12 IDA RGD PMID:19525015 RGD:2311085 NCBI chr15:1...55,302,115 JBrowse link
Q Neuinf8 Neuroinflammation QTL 8 IDA RGD PMID:19525015 RGD:2311085 NCBI chr 1:57,336,763...151,090,257 JBrowse link
Q Neuinf9 Neuroinflammation QTL 9 IDA RGD PMID:19525015 RGD:2311085 NCBI chr10:5,801,990...62,146,030 JBrowse link
Term paths to the root
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Term Annotations click to browse term
  measurement method 2368
    ex vivo method 1236
      enzymatic method 85
        polymerase chain reaction assay 5
          real-time polymerase chain reaction assay + 5
          reverse transcription polymerase chain reaction assay + 0
Path 2
Term Annotations click to browse term
  measurement method 2368
    molecular method 34
      expression assay 12
        transcript expression assay 5
          polymerase chain reaction assay 5
            real-time polymerase chain reaction assay + 5
            reverse transcription polymerase chain reaction assay + 0
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