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MEASUREMENT METHODS - ANNOTATIONS

The Clinical Measurement Ontology (CMO), Measurement Methods Ontology (MMO), and Experimental Condition Ontology (XCO) are currently being developed at the Rat Genome Database. For more information about these vocabularies please see Shimoyama et al. Three ontologies to define phenotype measurement data. Front Genet. 2012;3:87. Epub 2012 May 28 or contact us (https://rgd.mcw.edu/rgdweb/contact/contactus.html).
Term:bicinchoninic acid protein assay
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Accession:MMO:0000424 term browser browse the term
Definition:The BCA protein assay combines the protein-induced biuret reaction in which peptide bonds in the protein reduce Cu2+ ions to Cu+ in a reaction proportional to the amount of protein present, followed by chelation of bicinchoninic acid molecules with Cu+ ions, forming a purple-colored product which can be quantitated by spectrophotometry, i.e., based on the ability of the solution to alter the transmission of light at specific wavelengths in the visible spectrum, that is, to alter the transmission of a specific color of light.
Synonyms:related_synonym: BCA protein assay


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Related Phenotype Data for Term "bicinchoninic acid protein assay" (MMO:0000424)

Rat Strains:
Clinical Measurements:
Experimental Conditions:
Measurement Methods:
Term paths to the root
Path 1
Term Annotations click to browse term
  measurement method 2368
    ex vivo method 1236
      spectrometry 56
        spectrophotometry 21
          protein analysis by liquid phase colorimetry with spectrophotometric readout 9
            bicinchoninic acid protein assay 0
Path 2
Term Annotations click to browse term
  measurement method 2368
    ex vivo method 1236
      spectrometry 56
        photometry 40
          colorimetry 40
            liquid phase colorimetry 36
              peptide/protein analysis by liquid phase colorimetry 10
                protein analysis by liquid phase colorimetry with spectrophotometric readout 9
                  bicinchoninic acid protein assay 0
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