http://rgd.mcw.edu/wg/strain-maintenance/untitled http://rgd.mcw.edu/wg/strain-maintenance/untitled/what-is-a-sensitive-elisa-kit-for-detecting-estradiol-and-testosterone-in-the-blood-of-rats Diagnostic Products Corps's Coat-A-Count testosterone RIA kit (marketed for human blood/urine) has been used by some groups with success. For estradiol, Caymen Chemical's Estradioal EIA kit has been suggested. http://rgd.mcw.edu/wg/strain-maintenance/untitled/what-is-a-sensitive-elisa-kit-for-detecting-estradiol-and-testosterone-in-the-blood-of-rats Mon, 24 Mar 2008 16:30:50 -0500 http://rgd.mcw.edu/wg/strain-maintenance/untitled/what-are-the-dose-of-hormones-and-timing-needed-for-inducing-superovulation-in-rat-females 20 IU PMSG and 30 IU HCG 48 hours apart has been used successfully. See Filipiak WE, Saunders TL 2006, Advances in transgenic rat production. Transgenic Res. 15:673-686. http://rgd.mcw.edu/wg/strain-maintenance/untitled/what-are-the-dose-of-hormones-and-timing-needed-for-inducing-superovulation-in-rat-females Mon, 24 Mar 2008 16:29:56 -0500 http://rgd.mcw.edu/wg/strain-maintenance/untitled/how-do-you-differentiate-the-sex-of-neonatal-rats One way is to examine the ano-genital distance which will be longer in males than females. http://rgd.mcw.edu/wg/strain-maintenance/untitled/how-do-you-differentiate-the-sex-of-neonatal-rats Mon, 24 Mar 2008 16:28:57 -0500 http://rgd.mcw.edu/wg/strain-maintenance/untitled/what-anesthetics-affect-the-gaba-ergic-system GABA-ergic mechanisms are affected by barbiturates, benzodiazepines, propofol and steriod anesthetics and indirectly by volatile (gas) anesthetics. Some researchers have found success with Avertin(tribomoethanol). http://rgd.mcw.edu/wg/strain-maintenance/untitled/what-anesthetics-affect-the-gaba-ergic-system Mon, 24 Mar 2008 16:27:54 -0500